A simple methodology for rate-limiting step determination for anaerobic digestion of complex substrates and effect of microbial community ratio.

@article{Ma2013ASM,
  title={A simple methodology for rate-limiting step determination for anaerobic digestion of complex substrates and effect of microbial community ratio.},
  author={Jingwei Ma and Craig S Frear and Zhi-wu Wang and Liang Yu and Quanbao Zhao and Xiujin Li and Shulin Chen},
  journal={Bioresource technology},
  year={2013},
  volume={134},
  pages={391-5}
}
Anaerobic digestion (AD) of complex substrates is a multi-step process, which is kinetically controlled by an individual rate-limiting step. A methodology for determining the rate-limiting step during AD of complex substrates was developed by supplementation of metabolic intermediates from each digestion step with dairy manure as an emblematic complex substrate. This method elucidated that hydrolysis of dairy manure was the rate-limiting step when normal anaerobic sludge was used as inoculum… CONTINUE READING