A simple method using 31P-NMR spectroscopy for the study of protein phosphorylation.

Abstract

Nonradioactive 31P-NMR spectroscopy has previously been used for the study of protein phosphorylations. However, the procedures does not seem to be easy for non-experts of this field, hence, this approach has not been widely used. We introduce here a simple protocol with 31P-NMR spectroscopy to study in vitro phosphorylation in receptor proteins. The… (More)

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Cite this paper

@article{Hirai2000ASM, title={A simple method using 31P-NMR spectroscopy for the study of protein phosphorylation.}, author={Hirohisha Hirai and K. Yoshioka and K. Yamada}, journal={Brain research. Brain research protocols}, year={2000}, volume={5 2}, pages={182-9} }