Corpus ID: 45935285

A simple method of producing a monolayer of cervical cells for digital image processing.

@article{Rosenthal1979ASM,
  title={A simple method of producing a monolayer of cervical cells for digital image processing.},
  author={Dorothy L. Rosenthal and E Stern and C McLatchie and Ai Wu and Leo D. Lagasse and Ray J. Wall and Kenneth R. Castleman},
  journal={Analytical and quantitative cytology},
  year={1979},
  volume={1 2},
  pages={
          84-8
        }
}
A manual method of producing a monolayer of isolated and evenly distributed cervical epithelial cells has been developed for use in an automated digital image analysis system. The cervical scrape specimen is vortexed from the spatula into a solution of Mucosol. The suspension is filtered through a 10 micro nylon mesh to remove polys and debris and then syringed to disaggregate epithelial clumps. The cells are plated out on a Nuclepore filter. The filter is touched directly onto a glass… Expand
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