A simple method of preparing diverse neoagaro-oligosaccharides with beta-agarase.

@article{Li2007ASM,
  title={A simple method of preparing diverse neoagaro-oligosaccharides with beta-agarase.},
  author={Jingbao Li and Feng Han and Xinzhi Lu and Xiaoyan Fu and Cuiping Ma and Yan Ting Mandy Chu and Wengong Yu},
  journal={Carbohydrate research},
  year={2007},
  volume={342 8},
  pages={
          1030-3
        }
}
In order to prepare pure and well-defined oligosaccharides from agarose in a rapid and simple manner, an enzymatic degradation method was developed, which includes degradation with either recombinant beta-agarase (EC 3.2.1.81) AgaA or AgaB and gel permeation chromatography. Agarose was degraded with AgaA at the optimized conditions, yielding 47% and 45% of neoagarotetraose and neoagarohexaose, respectively. These neoagaro-oligosaccharides were conveniently separated by consecutive column… CONTINUE READING