A simple method of in vitro electroporation allows visualization, recording, and calcium imaging of local neuronal circuits.

@article{Hovis2010ASM,
  title={A simple method of in vitro electroporation allows visualization, recording, and calcium imaging of local neuronal circuits.},
  author={Kenneth Ray Hovis and Krishnan Padmanabhan and Nathaniel N. Urban},
  journal={Journal of neuroscience methods},
  year={2010},
  volume={191 1},
  pages={1-10}
}
Since Cajal's early drawings, the characterization of neuronal architecture has been paramount in understanding neuronal function. With the development of electrophysiological techniques that provide unprecedented access to the physiology of these cells, experimental questions of neuronal function have also become more tractable. Fluorescent tracers that can label the anatomy of individual or populations of neurons have opened the door to linking anatomy with physiology. Experimentally however… CONTINUE READING
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