A simple method for the detection of CYP2C9 polymorphisms: nested allele-specific multiplex polymerase chain reaction.

@article{Zainuddin2003ASM,
  title={A simple method for the detection of CYP2C9 polymorphisms: nested allele-specific multiplex polymerase chain reaction.},
  author={Z Zainuddin and Lay Kek Teh and Aisyah Suhaimi and Mohd Zaki Salleh and Rusli Ismail},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={2003},
  volume={336 1-2},
  pages={97-102}
}
BACKGROUND Cytochrome P4502C9 (CYP2C9), a principle drug-metabolizing enzyme is polymorphic in humans and is responsible for important pharmacokinetic and pharmacodynamic variations of CYP2C9 substrates. We developed an allele-specific multiplex polymerase chain reaction (PCR) method for the detection of common CYP2C9 alleles. METHOD Genomic DNA was extracted from blood obtained from 40 unrelated healthy Malaysian Indian volunteers. The DNA was subjected to a first PCR that was used to… CONTINUE READING