A simple method for preparation of snake venom phosphodiesterase almost free from 5'-nucleotidase.

Abstract

A simple method, involving NAD+-Sepharose chromatography, was developed for the preparation of snake venom phosphodiesterase (EC 3.1.4.1) almost free from 5'-Nucleotidase (EC 3.1.3.5). Using an NAD+-Sepharose 4B column, phosphodiesterase was eluted in the unadsorbed fraction, whereas 5'nucleotidase was strongly adsorbed. The latter enzyme was desorbed… (More)

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@article{Tatsuki1975ASM, title={A simple method for preparation of snake venom phosphodiesterase almost free from 5'-nucleotidase.}, author={Takao Tatsuki and Shiroh Iwanaga and T Suzuki}, journal={Journal of biochemistry}, year={1975}, volume={77 4}, pages={831-6} }