A simple method for organotypic cultures of nervous tissue.

Abstract

Hippocampal slices prepared from 2-23-day-old neonates were maintained in culture at the interface between air and a culture medium. They were placed on a sterile, transparent and porous membrane and kept in petri dishes in an incubator. No plasma clot or roller drum were used. This method yields thin slices which remain 1-4 cell layers thick and are… (More)

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Cite this paper

@article{Stoppini1991ASM, title={A simple method for organotypic cultures of nervous tissue.}, author={Luc Stoppini and P A Buchs and Dominique Muller}, journal={Journal of neuroscience methods}, year={1991}, volume={37 2}, pages={173-82} }