A simple method for obtaining high plating efficiencies with cultured insect cells

@article{Koval2005ASM,
  title={A simple method for obtaining high plating efficiencies with cultured insect cells},
  author={Thomas M. Koval},
  journal={Experientia},
  year={2005},
  volume={34},
  pages={674-676}
}
A technique has been developed for obtaining absolute plating efficiencies as high as 79% for culturedTrichoplusia ni (TN-368) cells. The method involves the use of conditioned medium and MES, BES, or ACES buffers. Cell growth and morphology are not altered under these conditions.