A simple method for maintaining large, aging populations of Caenorhabditis elegans 

@article{Gandhi1980ASM,
  title={A simple method for maintaining large, aging populations of Caenorhabditis elegans
},
  author={Schiva Gandhi and John Santelli and David Hillard Mitchell and J W Stiles and D. Rao Sanadi},
  journal={Mechanisms of Ageing and Development},
  year={1980},
  volume={12},
  pages={137-150}
}
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113 Citations
Highly Influential Citations
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Background Citations
35
Methods Citations
15
Results Citations
1

113 Citations

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Citation Type

Real-time egg laying dynamics in Caenorhabditis elegans
TLDR
A liquid culture system to deliver and remove food and pharmacological agents from individually housed worms and counts progeny in real-time, measuring embryo size as well as allowing for embryo recovery from individual mothers for further developmental assays is demonstrated.
Age-synchronous culture of Caenorhabditis elegans: technique and applications
Applications of Cold Temperature Stress to Age Fractionate Caenorhabditis elegans: A Simple Inexpensive Technique
TLDR
Cold temperature stress of mixed populations of CE provides a rapid inexpensive means of obtaining three life stage–specific cohorts that allow acquisition of replicate metabolite profiles of changes associated with development, aging, and senescence.
The biology of death in Caenorhabditis elegans
TLDR
This first description of the biology of death in C. elegans implies that the passage from life to death entails a regulated, programmed transition that is amenable to analysis.
Arresting development arrests aging in the nematode Caenorhabditis elegans
Programmed aging or error catastrophe? An exmination by two-dimensional polyacrylamide gel electrophoresis
Dramatic age-related changes in nuclear and genome copy number in the nematode Caenorhabditis elegans
TLDR
Both systematic loss of nuclei or nuclear DNA, as well as dramatic age‐related changes in nuclear genome copy number are reported and it is proposed that these changes are important pathobiological characteristics of aging nematodes.
Metabolism and aging in the nematode Caenorhabditis elegans.
The influence of metabolic rate on longevity in the nematode Caenorhabditis elegans.
TLDR
Comparisons of metabolic rate between long-lived and wild-type C. elegans under more optimized conditions indicate that the extended longevity of at least some long-living C. nematode mutants may be due to a reduction in metabolic rate, rather than an alteration of a metabolically independent genetic mechanism specific to aging.
The influence of metabolic rate on longevity in the nematode Caenorhabditis elegans *
TLDR
Comparisons of metabolic rate between long‐lived and wild‐type C. elegans under more optimized conditions indicate that the extended longevity of at least some long-lived C. aristans mutants may be due to a reduction in metabolic rate, rather than an alteration of a metabolically independent genetic mechanism specific to aging.
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References

SHOWING 1-10 OF 18 REFERENCES
Synchronous growth and aging of Caenorhabditis elegans in the presence of fluorodeoxyuridine.
TLDR
Treatment with FUdR under carefully controlled conditions is a reasonable way to maintain synchronously aging populations of C. elegans, and age-associated changes seen in controls also occurred in F UdR-treated worms, including atrophy of the gonads, increased pigmentation, sluggishness and increased transparency.
The life cycle of the nematode Caenorhabditis elegans. I. Wild-type growth and reproduction.
The nematode Caenorhabditis elegans: a new organism for intensive biological study.
TLDR
The nematode Caenorhabditis elegans may, according to some workers, become the Escherichia coli or at least the bacteriophage T4 of the animal world.
Aging in the free-living nematode Turbatrix aceti. Techniques for synchronization and aging of large-scale axenic cultures
Post-embryonic cell lineages of the nematode, Caenorhabditis elegans.
Practical methods for the axenic culture of the free-living nematodes Turbatrix aceti and Caenorhabditis briggsae.
  • M. Rothstein
  • Biology
    Comparative biochemistry and physiology. B, Comparative biochemistry
  • 1974
Aging in Caenorhabditis Briggsae
TLDR
Results suggest that the ability to osmoregulate and the permeability of the body wall are altered during senescence, which is probably associated with deterioration of the hypodermis organelles.
Aging in the nematode Caenorhabditis elegans: Major biological and environmental factors influencing life span
  • M. Klass
  • Biology
    Mechanisms of Ageing and Development
  • 1977
Temperature-sensitive developmental mutants of Caenorhabditis elegans.
Studies on aging in Nematodes. I. The nematode as a model organism for aging research.
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