A simple introduction to multiphoton microscopy.

  title={A simple introduction to multiphoton microscopy.},
  author={Alessandro Ustione and David W. Piston},
  journal={Journal of microscopy},
  volume={243 3},
Multiphoton microscopy is a powerful technique based on complex quantum mechanical effects. Thanks to the development of turnkey mode-locked laser systems, multiphoton microscopy is now available for everyone to use without extreme complexity. In this short introduction, we describe qualitatively the important concepts underlying the most commonly used type of multiphoton microscopy (two-photon excitation). We elucidate how those properties lead to the powerful results that have been achieved… CONTINUE READING

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Chapter 28: Multi-photon molecular excitation in laser-scanning microscopy

  • W. Denk, D. W. Piston, W. W. Webb
  • Handbook of Biological Confocal Microscopy
  • 2006
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