A simple in vitro RNA editing assay for chloroplast transcripts using fluorescent dideoxynucleotides: distinct types of sequence elements required for editing of ndh transcripts.

@article{Sasaki2006ASI,
  title={A simple in vitro RNA editing assay for chloroplast transcripts using fluorescent dideoxynucleotides: distinct types of sequence elements required for editing of ndh transcripts.},
  author={Tadamasa Sasaki and Yasushi Yukawa and Tatsuya Wakasugi and Kyoji Yamada and Masahiro Sugiura},
  journal={The Plant journal : for cell and molecular biology},
  year={2006},
  volume={47 5},
  pages={802-10}
}
RNA editing is found in various transcripts from land plant chloroplasts. In tobacco chloroplasts, C-to-U conversion occurs at 36 specific sites including two sites identified in this work. Our RNA editing assay system using chloroplast extracts facilitated biochemical analyses of editing reactions but required mRNAs labeled with (32)P at specific sites. Here, we have improved the in vitro system using fluorescence-labeled chain terminators, ddGTP and ddATP, and have measured the editing… CONTINUE READING
22 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 22 extracted citations

Similar Papers

Loading similar papers…