A simple image correction method for high-throughput microscopy

  title={A simple image correction method for high-throughput microscopy},
  author={Adam D. Coster and Chonlarat Wichaidit and Satwik Rajaram and Steven J Altschuler and Lani F Wu},
  journal={Nature Methods},
total or ratiometric intensities of biomarkers (Fig. 1a and Supplementary Note). For these simple features, background and shading can alter apparent phenotypic variability in surprising ways. For example, the effects of either on the ratiometric feature are generally unpredictable, whereas background increases total feature variability owing to underlying differences in cell size. Such changes to distributions of single-cell features can be particularly important in screening, where variation… CONTINUE READING