A simple hybridoma screening method for high-affinity monoclonal antibodies using the signal ratio obtained from time-resolved fluorescence assay.

Abstract

In hybridoma screening, quantitative kinetic evaluation is difficult since the concentration of each antibody in the hybridoma supernatant is unknown. From modeling calculations, we hypothesized that the ratio of two different antigen-antibody concentrations might allow discrimination of high-affinity monoclonal antibodies irrespective of the antibody… (More)

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Cite this paper

@article{Daigo2006ASH, title={A simple hybridoma screening method for high-affinity monoclonal antibodies using the signal ratio obtained from time-resolved fluorescence assay.}, author={Kenji Daigo and Sui Sugita and Yasuhiro Mochizuki and Hiroko Iwanari and Kanae Hiraishi and Kenjiro Miyano and Tatsuhiko Kodama and Takao Hamakubo}, journal={Analytical biochemistry}, year={2006}, volume={351 2}, pages={219-28} }