A simple fluorescence method for the study of the internalization of particles in cultured cells: Application to asbestos and glass fibres.

  title={A simple fluorescence method for the study of the internalization of particles in cultured cells: Application to asbestos and glass fibres.},
  author={Svein-Ole Mikalsen and K R Modalsli},
  journal={Toxicology in vitro : an international journal published in association with BIBRA},
  volume={8 2},
  • S. Mikalsen, K. Modalsli
  • Published 1 April 1994
  • Biology, Medicine
  • Toxicology in vitro : an international journal published in association with BIBRA
A model for the study of internalization of particles in mammalian cells was applied to asbestos and glass fibres. Briefly, a fluorescent fluid-phase endocytic marker, Lucifer Yellow CH (LY), was allowed to be incorporated into the lysosomal compartment of Syrian hamster embryo cells. Mineral fibres that were internalized by the cells subsequently became 'fluorescent', presumably when the fibre-containing endosome fused with the LY-containing lysosomes. This method was compared with… Expand
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