A simple fluorescence method for the study of the internalization of particles in cultured cells: Application to asbestos and glass fibres.

@article{Mikalsen1994ASF,
  title={A simple fluorescence method for the study of the internalization of particles in cultured cells: Application to asbestos and glass fibres.},
  author={Svein-Ole Mikalsen and K R Modalsli},
  journal={Toxicology in vitro : an international journal published in association with BIBRA},
  year={1994},
  volume={8 2},
  pages={
          243-50
        }
}
  • S. Mikalsen, K. Modalsli
  • Published 1 April 1994
  • Biology, Medicine
  • Toxicology in vitro : an international journal published in association with BIBRA
A model for the study of internalization of particles in mammalian cells was applied to asbestos and glass fibres. Briefly, a fluorescent fluid-phase endocytic marker, Lucifer Yellow CH (LY), was allowed to be incorporated into the lysosomal compartment of Syrian hamster embryo cells. Mineral fibres that were internalized by the cells subsequently became 'fluorescent', presumably when the fibre-containing endosome fused with the LY-containing lysosomes. This method was compared with… Expand
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References

SHOWING 1-10 OF 41 REFERENCES
Role of phagocytosis in Syrian hamster cell transformation and cytogenetic effects induced by asbestos and short and long glass fibers.
TLDR
Fiber length appears to affect the phagocytosis of fibers as well as the ability of intracellular fibers to induce cytogenetic damage and the resultant transformation in Syrian hamster embryo cells in culture. Expand
Characterization of the early endosome and putative endocytic carrier vesicles in vivo and with an assay of vesicle fusion in vitro
TLDR
The authors' observations suggest that the spherical vesicles mediate transport between the early endosome and late endosomes and that this process requires intact microtubules. Expand
Crocidolite asbestos fibers undergo size-dependent microtubule-mediated transport after endocytosis in vertebrate lung epithelial cells.
TLDR
It is concluded that, upon incorporation into epithelial cells, asbestos fibers undergo size-dependent active transport along cytoplasmic microtubules, the first to link the dimension-dependent transforming ability of asbestos fibers to a basic cellular function, i.e., the microtubule-dependent transport of cellular components. Expand
Chinese hamster ovary cell lysosomes rapidly exchange contents
TLDR
The experiments suggest that in vivo the lysosome is a rapidly intermixing organellar compartment that is as rapid, if not more rapid, than endosome/lysosome exchange. Expand
Mechanisms of cytotoxicity of asbestos fibres in rat tracheal epithelial cells in culture.
TLDR
A potentially important pathway of fibre toxicity involving binding, phagocytosis, nuclear damage, disruption of mitosis, inhibition of proliferation and/or cell death is suggested, since chrysotile and crocidolite fibres of similar length and diameter exhibit very different degrees of toxicity. Expand
Morphological transformation of Syrian hamster embryo cells induced by mineral fibres and the alleged enhancement of benzo[a]pyrene.
TLDR
It could be concluded that induction of transformation is not caused by unspecific cytotoxic effects, and no synergistic effect was observed between benzo[a]pyrene (BaP) and asbestos fibres. Expand
Translocation and clustering of endosomes and lysosomes depends on microtubules
TLDR
It can be concluded that translocation of endosomes and lysosomes occurs along microtubules and is independent of the intermediate filament and microfilament networks. Expand
pH-induced microtubule-dependent redistribution of late endosomes in neuronal and epithelial cells
The interaction between late endocytic structures and microtubules in polarized cells was studied using a procedure previously shown to cause microtubule-dependent redistribution of lysosomes inExpand
Actin microfilaments play a critical role in endocytosis at the apical but not the basolateral surface of polarized epithelial cells
TLDR
There is a fundamental difference in the process by which endocytic vesicles are formed at the two surfaces of polarized epithelial cells and that the integrity and/or the polymerization of actin filaments are required at the apical surface. Expand
Animal cell lysosomes rapidly exchange membrane proteins.
  • Y. Deng, B. Storrie
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1988
TLDR
The interspecies cell fusion/monoclonal antibody approach developed here should be readily applicable to determining if membrane protein exchange is a property of other organelles such as Golgi apparatus and mitochondria. Expand
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