A simple fluorescence labeling method for studies of protein oxidation, protein modification, and proteolysis.

Abstract

Proteins are sensitive to oxidation, and oxidized proteins are excellent substrates for degradation by proteolytic enzymes such as the proteasome and the mitochondrial Lon protease. Protein labeling is required for studies of protein turnover. Unfortunately, most labeling techniques involve (3)H or (14)C methylation, which is expensive, exposes researchers… (More)
DOI: 10.1016/j.freeradbiomed.2011.08.018

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