A simple approach to spectrally resolved fluorescence and bright field microscopy over select regions of interest

Abstract

A standard wide field inverted microscope was converted to a spatially selective spectrally resolved microscope through the addition of a polarizing beam splitter, a pair of polarizers, an amplitude-mode liquid crystal-spatial light modulator, and a USB spectrometer. The instrument is capable of simultaneously imaging and acquiring spectra over user defined… (More)

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Cite this paper

@inproceedings{Dahlberg2016ASA, title={A simple approach to spectrally resolved fluorescence and bright field microscopy over select regions of interest}, author={Peter D. Dahlberg and Christopher T. Boughter and Nabil F. Faruk and Lu Hong and Young Hoon Koh and Matthew A. Reyer and Alon Shaiber and Aiman Sherani and Jiacheng Zhang and Justin E. Jureller and Adam Hammond}, booktitle={The Review of scientific instruments}, year={2016} }