• Corpus ID: 24760040

A simple approach to single-cell microdissection and molecular analysis.

@article{Zhang1997ASA,
  title={A simple approach to single-cell microdissection and molecular analysis.},
  author={Z Zhang and M. Nakamura and Emiko Taniguchi and Lu-juan Shan and Toyoharu Yokoi and Kennichi Kakudo},
  journal={Analytical and quantitative cytology and histology},
  year={1997},
  volume={19 6},
  pages={
          514-8
        }
}
OBJECTIVE To develop a simple approach to single-cell microdissection from Papanicolaou-stained smears and to determine its usefulness in molecular diagnosis using archival cytologic material. STUDY DESIGN Culture cells from three cell lines (TT, A549 and Lu65) were used to prepare Papanicolaou-stained smears. Mount-Quick, a mounting medium, was used to establish the method of sorting target single cells from smear slides under direct microscopic observation. Calcitonin receptor gene and… 
Optimal molecular profiling of tissue and tissue components: defining the best processing and microdissection methods for biomedical applications.
TLDR
This article reviews current methods for obtaining anatomically specific signals from molecules isolated from tissues, a basic requirement for productive linking of phenotype and genotype, and provides a detailed comparison of some current tissue microdissection technologies.
Optimal molecular profiling of tissue and tissue components: defining the best processing and microdissection methods for biomedical applications.
TLDR
This updated chapter reviews current methods for obtaining anatomically specific signals from molecules isolated from tissues, a basic requirement for productive linking of phenotype and genotype, and provides a detailed comparison of some current tissue microdissection technologies.
Optimal molecular profiling of tissue and tissue components
TLDR
This article reviews current methods for obtaining anatomically specific signals from molecules isolated from tissues, a basic requirement for productive linking of phenotype and genotype, and provides a detailed comparison of some current tissue microdissection technologies.
PCR amplification of DNA obtained from archived hematoxylin‐eosin– and giemsa‐stained breast cancer aspirates
TLDR
The results demonstrate that archived stained smears prepared for cytologic examinations can be used for molecular analyses by using a PCR amplification method and conclude that the application of molecular biology techniques to the existing archival smears may become a valuable tool to detect genetic changes in samples from breast cancer aspirates.
New developments in the analysis of gene expression
Abstract An understanding of the relationship between gene expression, protein expression and the influences of genetic responses upon gene function is vital before we can understand the complexity
Pancreatic Cancer
TLDR
The salient histopathologic and molecular features of precursor lesions of pancreatic cancer as well as methods that have proved to be useful within experimental studies are presented.
Koilocytic changes are not elicited by human papillomavirus genotypes with higher oncogenic potential
TLDR
Most common high‐risk types belonging to α‐9 and α‐7 genotypes appear to rarely induce koilocytic changes, which may provide additional useful information for predicting the risk of progression to high‐grade lesions.
Pancreatic cancer
TLDR
GPs play an important role in the palliation of patients with incurable disease, and the stage of disease at diagnosis has significant prognostic importance, and as such, early investigation and referral of Patients with suspicious symptoms is crucial.
Akute myeloische Leukämien
Die akute myeloische Leukamie (AML) ist eine Gruppe von malignen Erkrankungen, die durch das unkontrollierte Wachstum unreifer hamatologischer Vorlauferzellen (Blasten) bei gleichzeitiger Storung