A simple approach for the analysis of intracellular movement of oxidant-producing intracellular compartments in living human neutrophils

@article{Kobayashi2000ASA,
  title={A simple approach for the analysis of intracellular movement of oxidant-producing intracellular compartments in living human neutrophils},
  author={Toshihiro Kobayashi and Vadim S. Zinchuk and Teruhiko Okada and Hiroshi Wakiguchi and Takanobu Kurashige and Hiroyoshi Takatsuji and Harumichi Seguchi},
  journal={Histochemistry and Cell Biology},
  year={2000},
  volume={113},
  pages={251-257}
}
In human neutrophils, superoxide is generated primarily within specialized oxidant-producing intracellular compartments. The present study employs a simple methodological approach to evaluate the intracellular movement of these structures in living human neutrophils. Using a CCD camera system, we monitored fluorescence in cells loaded with the succinimidyl ester of dichlorodihydrofluorescein diacetate, which is non-fluorescent until oxidized by reactive oxygen species. Fluorescence-positive… CONTINUE READING
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tection of oxidant - producing sites in glutaraldehyde - fixed human neutrophils and eosinophils stimulated with phorbol myr - istate acetate

  • T Kobayashi, E GarciadelSaz, J Hendry, H Seguchi
  • Histochem J
  • 1999

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