A simple and sensitive cAMP phosphodiesterase assay using a modification of the Ba(OH)2-ZnSO4 precipitation method.

Abstract

A modification of the zinc-barium precipitation method is described for the measurement of phosphodiesterase activity. This method differs from the previous precipitation method in that it measures the appearance of the 5'-AMP product in the precipitate rather than the disappearance of the cAMP substrate from the supernatant. The method is simple, rapid… (More)

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