A simple and reproducible method for directed evolution: combination of random mutation with dITP and DNA fragmentation with endonuclease V.

@article{Wang2013ASA,
  title={A simple and reproducible method for directed evolution: combination of random mutation with dITP and DNA fragmentation with endonuclease V.},
  author={Zun Wang and Hai-yan Wang and Hong Feng},
  journal={Molecular biotechnology},
  year={2013},
  volume={53 1},
  pages={49-54}
}
An alternative method to combine mutagenesis PCR with dITP and fragmentation by endonuclease V for directed evolution was developed. In comparison to the routine protocol for directed evolution, dITP was used as mutation reagent in the mutagenesis PCR. Subsequently, the incorporated dITP in the PCR products could represent as being the target of endonuclease V. Finally, the mutated dsDNA was fragmented by endonuclease V and then shuffled via assembly and reamplification as is usually done. In… CONTINUE READING

References

Publications referenced by this paper.
Showing 1-10 of 28 references

Advances in laboratory evolution of enzymes.

Current opinion in chemical biology • 2008
View 1 Excerpt

Laboratory-directed protein evolution.

Microbiology and molecular biology reviews : MMBR • 2005
View 1 Excerpt

Modeling the Effects of prl mutations on the Escherichia coli SecY complex

M. A. Smith, W. M. Clemons, Jr., C. J. DeMars, A. M. Flower
Journal of Bacteriology, • 2005
View 1 Excerpt

Similar Papers

Loading similar papers…