A simple and reliable pretreatment protocol facilitates fluorescent in situ hybridisation on tissue microarrays of paraffin wax embedded tumour samples.

@article{Chin2003ASA,
  title={A simple and reliable pretreatment protocol facilitates fluorescent in situ hybridisation on tissue microarrays of paraffin wax embedded tumour samples.},
  author={S-F Chin and Yataro Daigo and He Huang and N Gopalakrishna Iyer and Grace Callagy and T Kranjac and Macarena Gonz{\'a}lez and Trogon Sangan and Helena Margaret Earl and C Caldas},
  journal={Molecular pathology : MP},
  year={2003},
  volume={56 5},
  pages={
          275-9
        }
}
AIMS To describe a robust pretreatment protocol for preparing paraffin wax embedded tissues on tissue microarrays for fluorescence in situ hybridisation (FISH). The newly developed pretreatment protocol described here was compared with the commonly used sodium thiocyanate based protocol and two different heating methods used in standard antigen unmasking protocols for immunohistochemistry (pressure cooking and microwaving in citrate acid buffer). METHODS Dewaxed tissue sections were incubated… CONTINUE READING

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