A simple and reliable method of producing in vitro infections of Cryptosporidium parvum (Apicomplexa).

@article{Upton1994ASA,
  title={A simple and reliable method of producing in vitro infections of Cryptosporidium parvum (Apicomplexa).},
  author={Steve J. Upton and Matt Tilley and Michael V. Nesterenko and D B Brillhart},
  journal={FEMS microbiology letters},
  year={1994},
  volume={118 1-2},
  pages={45-9}
}
A variety of techniques have been used to infect cell monolayers in culture with the protozoan, Cryptosporidium parvum. However, most of these methods rely on the use of trypsin and/or bile salts to excyst sporozoites in vitro, followed by washing sporozoites free of excystation solution prior to their addition to subconfluent monolayers. This method not… CONTINUE READING