A simple and rapid method for the determination of "free" iron in biological fluids.

@article{Nilsson2002ASA,
  title={A simple and rapid method for the determination of "free" iron in biological fluids.},
  author={Ulf S. Nilsson and Martina Bassen and Karin S{\"a}vman and Ingemar Kjellmer},
  journal={Free radical research},
  year={2002},
  volume={36 6},
  pages={677-84}
}
We present a convenient method for determining "free" or non-protein-bound iron in biological fluids. The new method is based on the bathophenantroline method for determination of total serum iron, and comprises binding of iron by a chromogenic chelator (bathophenantroline-disulphonate, BPS), which is specific for ferrous iron. The ferrous complex of BPS absorbs strongly at 535 nm, and the detection limit is less than 1 microM in a sample size of 50 microliters. The chelator does not liberate… CONTINUE READING

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