A simple and rapid method for intra- and interspecific transformation of Bacillus subtilis on solid media by DNA in protoplast lysates.

@article{Akamatsu2001ASA,
  title={A simple and rapid method for intra- and interspecific transformation of Bacillus subtilis on solid media by DNA in protoplast lysates.},
  author={Takashi Akamatsu and Hayao Taguchi},
  journal={Bioscience, biotechnology, and biochemistry},
  year={2001},
  volume={65 2},
  pages={446-8}
}
A simple method for intra- and interspecific transformation of Bacillus subtilis on solid media has been devised with DNA in protoplast lysates, 0.8% agar, glutamate, and yeast extract. The transformation frequency is 2.3 x 10(3) transformants per microg DNA, 10-20 times higher than that for conventional transformation on solid media. The method can be applicable to transformation in microtiter plates.