A simple and quantitative purification of glycosphingolipids and phospholipids by thin-layer chromatography blotting.

@article{Taki1994ASA,
  title={A simple and quantitative purification of glycosphingolipids and phospholipids by thin-layer chromatography blotting.},
  author={Takao Taki and Takeshi Kasama and Shizuo Handa and Dai Ishikawa},
  journal={Analytical biochemistry},
  year={1994},
  volume={223 2},
  pages={232-8}
}
A new and simple method for purifying glycosphingolipids and phospholipids by using "TLC blotting" was established. Glycosphingolipids separated by two-dimensional thin-layer chromatography (TLC) were made visible with primuline reagent, and then bands were marked with a drawing colored pencil. The glycosphingolipids that separated on the HPTLC plate were transferred by TLC blotting to a polyvinylidene difluoride membrane together with the color marks. The marked areas were excised after which… CONTINUE READING

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