A simple and efficient method for microdissection and microFISH.

@article{Engelen1998ASA,
  title={A simple and efficient method for microdissection and microFISH.},
  author={J. J. Engelen and Jozefa C M Albrechts and Guus J. H. Hamers and Joep P. M. Geraedts},
  journal={Journal of medical genetics},
  year={1998},
  volume={35 4},
  pages={265-8}
}
A simple and efficient method for the dissection of (marker) chromosomes, (micro)nuclei, and chromosome regions is presented. Before microdissection, metaphases are overlaid with milli-Q water to rehydrate the chromosomes, which makes them soft and sticky. The dissected chromosome fragments are dissolved without proteinase-K or topoisomerase treatment and directly amplified using a degenerate oligonucleotide primed polymerase chain reaction (DOP-PCR). The advantages of this microFISH method… CONTINUE READING

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