A simple and defined method to study calcification by isolated matrix vesicles. Effect of ATP and vesicle phosphatase.

@article{Hsu1977ASA,
  title={A simple and defined method to study calcification by isolated matrix vesicles. Effect of ATP and vesicle phosphatase.},
  author={Howard H T Hsu and H. Clarke Anderson},
  journal={Biochimica et biophysica acta},
  year={1977},
  volume={500 1},
  pages={
          162-72
        }
}
A simplified and defined system was developed to study in vitro calcium phosphate deposition by isolated matrix vesicles from rabbit growth plate cartilage, and to examine the relationship between vesicle phosphatase and calcium deposition. Samples of suspended vesicles containing 25 microgram of protein, were incubated for 2 h in a 45Ca-labelled solution with 2.2 mM Ca2+, 1.6 mM PO 3/4-and 1 mM ATP at pH 7.6. Calcium deposition was related to the amount of PO4 hydrolysed by matrix vesicle… CONTINUE READING

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Calcium incorporation in matrix vesicles isolated from chicken epiphyseal cartilage

  • Calcified Tissue International
  • 1980
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  • Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists
  • 2000