A simple PCR procedure for discovering microsatellites from small insert libraries

@inproceedings{Wang2007ASP,
  title={A simple PCR procedure for discovering microsatellites from small insert libraries},
  author={Xinwang Wang and Robert N Trigiano and Mark T. Windham and Renae Erin DeVries and Brian E Scheffler and Timothy A. Rinehart and James M. Spiers},
  year={2007}
}
Microsatellite discovery from genomic libraries is tedious because of the low number of clones that contain inserts and costly because of screening methodologies. A new procedure for screening clones for microsatellite DNA is described herein. Instead of colony hybridization, a polymerase chain reaction (PCR) with two vector standard primers and one synthesized repeat primer was used to directly screen colonies. PCR of colonies that produced a strong smear in gels contained the desired motif… CONTINUE READING
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