A simple Gateway-assisted construction system of TALEN genes for plant genome editing

@inproceedings{Kusano2016ASG,
  title={A simple Gateway-assisted construction system of TALEN genes for plant genome editing},
  author={Hiroaki Kusano and Hitomi Onodera and Miho Kihira and Hiromi Aoki and Hikaru Matsuzaki and Hiroaki Shimada},
  booktitle={Scientific reports},
  year={2016}
}
TALEN is an artificial nuclease being applied for sequence-specific genome editing. For the plant genome editing, a pair of TALEN genes is expressed in the cells, and a binary plasmid for Agrobacterium-mediated transformation should be assembled. We developed a novel procedure using the Gateway-assisted plasmids, named Emerald-Gateway TALEN system. We constructed entry vectors, pPlat plasmids, for construction of a desired TALEN gene using Platinum Gate TALEN kit. We also created destination… CONTINUE READING