A simple, single-step technique for selecting and cloning hybridomas for the production of monoclonal antibodies.

@article{Davis1982ASS,
  title={A simple, single-step technique for selecting and cloning hybridomas for the production of monoclonal antibodies.},
  author={Julianne M. Davis and Juck E. Pennington and A M Kubler and J F Conscience},
  journal={Journal of immunological methods},
  year={1982},
  volume={50 2},
  pages={161-71}
}
A simple method is described by which hybridomas can be selected and cloned in a single step immediately after fusion. This is done by plating the cells in semi-solid medium containing methylcellulose and the components of the HAT selection system. A number of variables have been examined in order to optimise the technique. The system is particularly… CONTINUE READING