A simple, reliable M'Fadyean stain for visualizing the Bacillus anthracis capsule.


The simple polychrome methylene blue (PMB) staining procedure for blood or tissue smears from dead animals (M'Fadyean reaction) established in 1903 remained accepted as a highly reliable, rapid diagnostic test for anthrax for six decades while that disease was still common in livestock throughout the world. Improvements in disease control led to anthrax… (More)
DOI: 10.1016/j.mimet.2013.01.009


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