A simple, rapid, and highly efficient gene expression system for multiheme cytochromes c.

@article{Ozawa2001ASR,
  title={A simple, rapid, and highly efficient gene expression system for multiheme cytochromes c.},
  author={Kiyoshi Ozawa and Fumiko Yasukawa and Yoko Fujiwara and Haruko Akutsu},
  journal={Bioscience, biotechnology, and biochemistry},
  year={2001},
  volume={65 1},
  pages={185-9}
}
The genes of tetraheme cytochrome c3 and hexadecaheme high-molecular-weight cytochrome c from Desulfovibrio vulgaris could be overexpressed as holoproteins in Shewanella oneidensis TSP-C using pUC-type vectors of E. coli. Surprisingly, S. oneidensis was transformed directly by pUC-type vectors through electroporation. The yields of the recombinant proteins in this expression system were much higher than the previously reported ones.