A simple, high-resolution method for establishing DNA binding affinity and sequence selectivity.

@article{Boger2001ASH,
  title={A simple, high-resolution method for establishing DNA binding affinity and sequence selectivity.},
  author={Dale L. Boger and Brian E Fink and Steven R Brunette and W. C. Tse and Michael P. Hedrick},
  journal={Journal of the American Chemical Society},
  year={2001},
  volume={123 25},
  pages={5878-91}
}
Full details of the development of a simple, nondestructive, and high-throughput method for establishing DNA binding affinity and sequence selectivity are described. The method is based on the loss of fluorescence derived from the displacement of ethidium bromide or thiazole orange from the DNA of interest or, in selected instances, the change in intrinsic fluorescence of a DNA binding agent itself and is applicable for assessing relative or absolute DNA binding affinities. Enlisting a library… CONTINUE READING
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