A simple, fast, one-step method for the purification of the skeletal-muscle ryanodine receptor.

Abstract

In this paper we describe a simple, fast, one-step method for the purification of the skeletal-muscle ryanodine receptor. The ryanodine receptor from CHAPS-solubilized junctional sarcoplasmic-reticulum membranes was adsorbed to a spermine-agarose column and eluted by 2 mM-spermine. The purified receptor, consisting predominantly of a 450 kDa polypeptide on… (More)

Topics

5 Figures and Tables

Slides referencing similar topics