A simple, RNA-mediated allosteric switch controls the pathway to formation of a T=3 viral capsid.

@article{Stockley2007ASR,
  title={A simple, RNA-mediated allosteric switch controls the pathway to formation of a T=3 viral capsid.},
  author={Peter G. Stockley and Ottar Rolfsson and Gary S. Thompson and Gabriella Basnak and S Francese and Nicola J Stonehouse and Steven W Homans and Alison E Ashcroft},
  journal={Journal of molecular biology},
  year={2007},
  volume={369 2},
  pages={541-52}
}
Using mass spectrometry we have detected both assembly intermediates and the final product, the T=3 viral capsid, during reassembly of the RNA bacteriophage MS2. Assembly is only efficient when both types of quasiequivalent coat protein dimer seen in the final capsid are present in solution. NMR experiments confirm that interconversion of these conformers is allosterically regulated by sequence-specific binding of a short RNA stem-loop. Isotope pulse-chase experiments confirm that all… CONTINUE READING