A silver staining method for single-cell gel assay.

@article{Nadin2001ASS,
  title={A silver staining method for single-cell gel assay.},
  author={Silvina Beatriz Nadin and Laura Mar{\'i}a Vargas-Roig and Daniel Ram{\'o}n Ciocca},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={2001},
  volume={49 9},
  pages={1183-6}
}
The single-cell gel assay (comet assay) is a very useful microelectrophoretic technique for evaluation of DNA damage and repair in individual cells. Usually, the comets are visualized and evaluated with fluorescent DNA stains. This staining requires specific equipment (e.g., a high-quality fluorescence microscope), the slides must be analyzed immediately, and they cannot be stored for long periods of time. Here we describe, using human lymphocytes, some modifications of the silver staining for… CONTINUE READING
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