A short story of aequorin.
@article{Shimomura1995ASS, title={A short story of aequorin.}, author={Osamu Shimomura}, journal={The Biological bulletin}, year={1995}, volume={189 1}, pages={ 1-5 } }
One day in the fall of 1960, shortly after my arrival at Princeton from Japan, Dr. Frank Johnson showed me a small jar containing a spoonful of white powder. He explained that the powder was a freeze dried “squeezate” made from the luminous jellyfish Aequorea, and that it would emit light when mixed with water. He asked me if I would be interested in studying the bioluminescence of this jellyfish. The powder did not emit any light when moistened. But I was quite impressed by Dr. Johnson’s…
61 Citations
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References
Calcium transients in single muscle fibers.
- BiologyBiochemical and biophysical research communications
- 1967