A short protocol for micro-purification of nuclear proteins from whole animal tissue.

@article{Roy1991ASP,
  title={A short protocol for micro-purification of nuclear proteins from whole animal tissue.},
  author={Robert J. Le Roy and Pierre Gosselin and Sylvain L. Gu{\'e}rin},
  journal={BioTechniques},
  year={1991},
  volume={11 6},
  pages={770-7}
}
Although a number of small-scale procedures have been described for the preparation of crude nuclear extracts from established cell lines, none were provided for the preparation of similar extracts from small amounts of animal tissue. In addition, no small-scale procedures contain enrichment steps that render the detection of low-abundant DNA-binding proteins easier. Here we describe a simple, efficient procedure for the rapid preparation of high-quality nuclear extracts from either whole… CONTINUE READING