A short history of blebbing

@article{Charras2008ASH,
  title={A short history of blebbing},
  author={Guillaume T. Charras},
  journal={Journal of Microscopy},
  year={2008},
  volume={231}
}
  • G. Charras
  • Published 1 September 2008
  • Biology
  • Journal of Microscopy
Blebs are protrusions of the cell membrane. They are the result of actomyosin contractions of the cortex, which cause either transient detachment of the cell membrane from the actin cortex or a rupture in the actin cortex. Then, cytosol streams out of the cell body and inflates the newly formed bleb. During expansion, which lasts ∼30 s, the bleb is devoid of actin and the surface area increases through further tearing of membrane from the cortex and convective flows of lipids in the plane of… 

A RhoA and Rnd3 cycle regulates actin reassembly during membrane blebbing

TLDR
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Membrane shrinkage and cortex remodelling are predicted to work in harmony to retract blebs

TLDR
It is demonstrated that cortex retraction alone cannot account for bleb retraction and suggested that the mechanism works in tandem with membrane shrinking, and an emergent hysteresis loop is observed in the intracellular pressure, which suggests a potential mechanism through which a secondary bleb can be initiated as a primary bleb contracts.

Membrane bleb: A seesaw game of two small GTPases

TLDR
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Assembly and composition of the cellular actin cortex

TLDR
It is discovered by localisation and silencing studies, that formin Diaph1 and the Arp2/3 complex are needed for the cortex nucleation, as both localised to the cortex and depletion of either led to cortical defects.

Interaction and fusion dynamics between cellular blebs.

Multiscale Computation of Cytoskeletal Mechanics During Blebbing

TLDR
A general multiscale interaction procedure is applied to the problem of cellular blebbing and is efficient enough to show cellular level effects produced by changes at the microscopic level, such as biochemical reaction rates.

abLIM1 constructs non-erythroid cortical actin networks to prevent mechanical tension-induced blebbing

TLDR
Actin-binding protein abLIM1 governs the formation of dense interconnected cortical actin meshwork in non-erythroid cells to prevent mechanical tension-induced blebbing during cellular activities such as spreading and migration.

Fluxes of Water through Aquaporin 9 Weaken Membrane-Cytoskeleton Anchorage and Promote Formation of Membrane Protrusions

TLDR
A model for AQP 9-induced membrane protrusion is presented, where the interplay of water fluxes through AQP9 and actin dynamics regulate the cellular protrusive and motile activity of cells.
...

References

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TLDR
The Rho pathway was important for cortex assembly in blebs, and Ezrin played no role in actin nucleation, but was essential for tethering the membrane to the cortex.

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TLDR
It is shown that bleb nucleation depends on pressure, membrane-cortex adhesion energy, and membrane tension, and is tested experimentally to find that retracting blebs are fivefold more rigid than expanding blebs, an increase entirely explained by the properties of the newly formed cortical actin mesh.

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TLDR
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TLDR
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TLDR
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TLDR
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