A short C-terminal sequence is necessary and sufficient for the targeting of chitinases to the plant vacuole.

@article{Neuhaus1991ASC,
  title={A short C-terminal sequence is necessary and sufficient for the targeting of chitinases to the plant vacuole.},
  author={James Neuhaus and Liliane Sticher and Frederick Meins and Thomas Boller},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1991},
  volume={88 22},
  pages={10362-6}
}
Tobacco contains different isoforms of chitinase (EC 3.2.1.14), a hydrolase thought to be involved in the defense against pathogens. Deduced amino acid sequences for putatively vacuolar, basic chitinases differ from the homologous extracellular, acidic isoforms by the presence of a C-terminal extension. To examine the role of this C-terminal extension in protein sorting, Nicotiana silvestris plants were stably transformed with chimeric genes coding for tobacco basic chitinase A with and without… CONTINUE READING
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