A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae.

@article{Gari1997ASO,
  title={A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae.},
  author={E. Noem{\'i} Gari and L{\'i}dia Piedrafita and Mart{\'i} Aldea and Enrique Montero Herrero},
  journal={Yeast},
  year={1997},
  volume={13 9},
  pages={837-48}
}
A set of Saccharomyces cerevisiae expression vectors has been developed in which transcription is driven by a hybrid tetO-CYC1 promoter through the action of a tetR-VP16 (tTA) activator. Expression from the promoter is regulated by tetracycline or derivatives. Various modalities of promoter and activator are used in order to achieve different levels of maximal expression. In the presence of antibiotic in the growth medium at concentrations that do not affect cell growth, expression from the… CONTINUE READING
Highly Influential
This paper has highly influenced 24 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 179 extracted citations

Similar Papers

Loading similar papers…