A set of lacZ mutations in Escherichia coli that allow rapid detection of specific frameshift mutations.

@article{Cupples1990ASO,
  title={A set of lacZ mutations in Escherichia coli that allow rapid detection of specific frameshift mutations.},
  author={Claire G. Cupples and Marta Cabrera and C. Cruz and J. H. Miller},
  journal={Genetics},
  year={1990},
  volume={125 2},
  pages={275-80}
}
We have used site-directed mutagenesis to alter bases in lacZ near the region encoding essential residues in the active site of beta-galactosidase. The altered sequences generate runs of six or seven identical base pairs which create a frameshift, resulting in a Lac- phenotype. Reversion to Lac+ in each strain can occur only by a specific frameshift at these sequences. Monotonous runs of A's (or of T's on the opposite strand) and G's (or C's) have been constructed, as has an alternating -C-G… CONTINUE READING
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