• Biology, Medicine
  • Published in Genetics 1990

A set of lacZ mutations in Escherichia coli that allow rapid detection of specific frameshift mutations.

@article{Cupples1990ASO,
  title={A set of lacZ mutations in Escherichia coli that allow rapid detection of specific frameshift mutations.},
  author={Claire G. Cupples and Marta Cabrera and C Cruz and J. H. Miller},
  journal={Genetics},
  year={1990},
  volume={125 2},
  pages={
          275-80
        }
}
We have used site-directed mutagenesis to alter bases in lacZ near the region encoding essential residues in the active site of beta-galactosidase. The altered sequences generate runs of six or seven identical base pairs which create a frameshift, resulting in a Lac- phenotype. Reversion to Lac+ in each strain can occur only by a specific frameshift at these sequences. Monotonous runs of A's (or of T's on the opposite strand) and G's (or C's) have been constructed, as has an alternating -C-G… CONTINUE READING

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References

Publications referenced by this paper.
SHOWING 1-10 OF 12 REFERENCES

A set of lacZ mutations in Escherichia coli which allow rapid detection of each of the six base substitutions

  • V. DAMAGNEZ, M.-P. DOUTRIAUX, M. RADMAN
  • Proc . Natl . Acad . Sci . USA
  • 1989

Sat - uration of mismatch repair in the mutD 5 mutator strain of Escherichia coli

  • R. WAGNER, M. RADMAN
  • J . Bacteriol .
  • 1989

Effects of amino acid substitutions at the active site in Escherichia coli @ - galactosidase

  • J. MILLER
  • 1988

Methyl-directed repair of frameshift mutations in heteroduplex DNA.

Detection of carcinogens as mutagens in the Salmonella microsome test : assay of 300 chemicals

  • H. MILLERJ.
  • Proc . Natl . Acad . Sci . USA
  • 1985

Genetic studies of the lac repressor . IV . Mutagenic specificity in the lac 1 gene of Escherichia coli

  • J. H. MILLER, P. J. FARABAUGH
  • J . Mol . Biol .
  • 1977