A series of IncQ-based reporter plasmids for use in a range of Gram negative genera.

@article{OSullivan2010ASO,
  title={A series of IncQ-based reporter plasmids for use in a range of Gram negative genera.},
  author={Laura E O'Sullivan and Cheryl A Nickerson and James T. Wilson},
  journal={Journal of microbiology and biotechnology},
  year={2010},
  volume={20 5},
  pages={871-4}
}
Many studies require expression analysis of the same gene/promoter across a range of bacterial genera. However, there is currently a lack of availability of reporters based on the broad host range IncQ replicon, which is compatible with a popular improved IncP transfer system that is self-transfer defective. We report IncQ lacZ reporter plasmids with features including: (1) compatibility with IncP, IncW, and pBHR/pBBR replicons, (2) a variety of antibiotic markers (Sp-r, Sm-r, Km-r, Cm-r), (3… CONTINUE READING