A sequence similar to tRNA3Lys gene is embedded in HIV-1 U3/R and promotes minus strand transfer

@inproceedings{PieknaPrzybylska2010ASS,
  title={A sequence similar to tRNA3Lys gene is embedded in HIV-1 U3/R and promotes minus strand transfer},
  author={Dorota Piekna-Przybylska and Laura DiChiacchio and David H. Mathews and Robert A Bambara},
  booktitle={Nature Structural &Molecular Biology},
  year={2010}
}
We identified a sequence embedded in the U3–R region of HIV-1 RNA that is highly complementary to human tRNA3Lys. The free energy of annealing to tRNA3Lys is significantly lower for this sequence and the primer-binding site than for other viral sequences of similar length. The only interruption in complementarity is a 29-nucleotide segment inserted where a tRNA intron would be expected. The insert contains the TATA box for viral RNA transcription. The embedded sequence includes a 9-nucleotide… CONTINUE READING