A sensitive transcriptome analysis method that can detect unknown transcripts.

@article{Fukumura2003AST,
  title={A sensitive transcriptome analysis method that can detect unknown transcripts.},
  author={Ryutaro Fukumura and Hirokazu Takahashi and Toshiyuki Saito and Yoko Tsutsumi and Akira Fujimori and Shinji Sato and Kouichi Tatsumi and Ryoko Araki and Masumi Abe},
  journal={Nucleic acids research},
  year={2003},
  volume={31 16},
  pages={
          e94
        }
}
We have developed an AFLP-based gene expression profiling method called 'high coverage expression profiling' (HiCEP) analysis. By making improvements to the selective PCR technique we have reduced the rate of false positive peaks to approximately 4% and consequently the number of peaks, including overlapping peaks, has been markedly decreased. As a result we can determine the relationship between peaks and original transcripts unequivocally. This will make it practical to prepare a database of… CONTINUE READING

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