A sensitive technique to monitor gene transfer and expression in bone marrow stem cells.

Abstract

The polymerase chain reaction technique (PCR), a primer-mediated enzymatic amplification of specific target sequences, was used to monitor gene transfer into hematopoietic progenitor stem cells. A gene coding for human interleukin 1 alpha (IL-1 alpha) was cotransfected with the rous sarcoma virus (RSV) CAT plasmid into mouse bone marrow cells by… (More)

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@article{Narayanan1989AST, title={A sensitive technique to monitor gene transfer and expression in bone marrow stem cells.}, author={Ramesh K. Narayanan and Nadine S Tare and William R. Benjamin and Ueli Gubler}, journal={Experimental hematology}, year={1989}, volume={17 7}, pages={832-5} }