A sensitive radiometric assay to measure D-xylulose kinase activity.

@article{Tritsch2004ASR,
  title={A sensitive radiometric assay to measure D-xylulose kinase activity.},
  author={Denis Tritsch and Andr{\'e}a Hemmerlin and Michel Rohmer and Thomas J. Bach},
  journal={Journal of biochemical and biophysical methods},
  year={2004},
  volume={58 1},
  pages={75-83}
}
A radiometric test system for D-xylulose kinase (XK) was developed for the measurement of enzyme activity in crude cell extracts and to minimize the volume of reaction mixtures besides increasing the sensitivity. [U-14C]xylulose 5-phosphate was produced from commercially available [U-14C]xylose in a coupled assay system containing D-xylose isomerase, which… CONTINUE READING