A sensitive radiometric assay for cysteic acid decarboxylase activity in crude enzyme preparations of rat liver and brain.

@article{Yam1977ASR,
  title={A sensitive radiometric assay for cysteic acid decarboxylase activity in crude enzyme preparations of rat liver and brain.},
  author={C. Yam and G. Tunnicliff and T. Ngo and A. Barbeau},
  journal={Comparative biochemistry and physiology. B, Comparative biochemistry},
  year={1977},
  volume={58 1},
  pages={
          115-7
        }
}
  • C. Yam, G. Tunnicliff, +1 author A. Barbeau
  • Published 1977
  • Chemistry, Medicine
  • Comparative biochemistry and physiology. B, Comparative biochemistry
  • 1. A method is described for the synthesis of L-[U-14C]cysteic acid from L-[U-14C] cysteine hydrochloride and for its subsequent utilisation as a substrate for cysteic acid decarboxylase activity in liver and brain. 2. The enzyme determination relies on the entrapment of radio-labelled carbon dioxide in Hyamine hydroxide. 3. The assay is sensitive, reliable and convenient and is particularly suitable for measuring the activity of the decarboxylase in crude enzyme preparations. 
    VITAMIN B-6 AND SULFUR AMINO ACID METABOLISM
    • 3

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