A sensitive non-radioactive northern blot method to detect small RNAs

@inproceedings{Kim2010ASN,
  title={A sensitive non-radioactive northern blot method to detect small RNAs},
  author={Sang Woo Kim and Zhihua Li and Patrick S Moore and A. Paula Monaghan and Yuan Chang and Mark Nichols and Bino John},
  booktitle={Nucleic acids research},
  year={2010}
}
The continuing discoveries of potentially active small RNAs at an unprecedented rate using high-throughput sequencing have raised the need for methods that can reliably detect and quantitate the expression levels of small RNAs. Currently, northern blot is the most widely used method for validating small RNAs that are identified by methods such as high-throughput sequencing. We describe a new northern blot-based protocol (LED) for small RNA (approximately 15-40 bases) detection using digoxigenin… CONTINUE READING
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